Last edited by Meshura
Sunday, May 3, 2020 | History

7 edition of Fluorescent Energy Transfer Nucleic Acid Probes found in the catalog.

Fluorescent Energy Transfer Nucleic Acid Probes

Designs And Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)

by Vladimir V. Didenko

  • 123 Want to read
  • 33 Currently reading

Published by Humana Press .
Written in English

    Subjects:
  • Genetics (non-medical),
  • Molecular biology,
  • Science/Mathematics,
  • Life Sciences - Cytology,
  • Science,
  • methods,
  • Life Sciences - Biochemistry,
  • Science / Cytology,
  • Life Sciences - Biology - Molecular Biology,
  • Fluorescence Resonance Energy Transfer,
  • Nucleic Acid Probes

  • The Physical Object
    FormatHardcover
    Number of Pages372
    ID Numbers
    Open LibraryOL8828476M
    ISBN 101588293807
    ISBN 109781588293800

    When a fluorescent dye is excited at a particular wavelength, it is promoted to an excited state. In the absence of a quencher, the excited dye emits light in returning to the ground state. When a quencher . 2. target probes anneal to nucleic acid and to preamplifier probes 3. amplifier probes anneal to preamplifier probes, forming a branched DNA structure 4. label probes (with bound alkaline .

    FLUORESCENCE RESONANCE ENERGY TRANSFER [ 18] [ 18] Fluorescence Resonance Energy T r a n s f e r and N u c l e i c Acids By ROBERT M. CLEGG Introduction Fluorescence resonance Cited by: Title: Fluorescent Nucleotide Base Analogs as Probes of Nucleic Acid Structure, Dynamics and Interactions VOLUME: 6 ISSUE: 9 Author(s):Manuela J. Rist and John P. Marino Affiliation:Center for Cited by:

    nucleic acid probe a labeled single-stranded nucleic acid molecule used to locate a specific nucleotide sequence in a nucleic acid sample. Moleucle of the rpobe hydrogen bodn to the complementary . Fluorescent hybridization probes for nucleic acid detection Jia Guo, Jingyue Ju, Nicholas J. Turro Analytical and Bioanalytical Chemistry (10), Cited by:


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Fluorescent Energy Transfer Nucleic Acid Probes by Vladimir V. Didenko Download PDF EPUB FB2

Exhaustive and state-of-the-art, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is an invaluable resource for both novice and experienced researchers who wish to use the latest.

"Fluorescence Energy Transfer Nucleic Acid Probes, Design and Protocols is a timely book. It is the first concise collection of fluorescence methods and assays used in exploring biomolecular structure.

Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the first publication to provide such a collection. In the volume, hands-on experts-often the original developers of the technique Price: $ Buy Fluorescent Energy Transfer Nucleic Acid Probes (): Designs and Protocols: NHBS - Edited By: Vladimir Didenko, Humana Press.

Book Review. Methods in Molecular Biology, Vol. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols. Edited by Vladimir V. Didenko. Sabine Müller. Ernst Moritz Arndt Universität. Labeling methods have been developed that control the location and number of fluorescent labels attached to a nucleic acid.

The ability to attach a fluorescent label at a specific Cited by: ECHO probes show absorption shift and emission switching depending on hybridization with the target nucleic acid.

The hybridization-sensitive fluorescence emission of ECHO probes and the further Cited by:   Hybridization of two fluorescent oligonucleotides to adjacent nucleic acid sequences led to fluorescence excitation energy transfer between the donor and the acceptor dyes.

This phenomenom Cited by: Because fluorescent hybridization probes are available in a wide range of colors, multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a Cited by: This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native Cited by:   Hybridization of two fluorescent oligonucleotides to adjacent nucleic acid sequences led to fluorescence excitation energy transfer between the donor and the acceptor dyes.

This phenomenon Cited by: Fluorescent hybridization probes based on the principles of fluorescence resonance energy transfer (FRET) and the complementary pairing of nucleic acids have been widely used in nucleic acid.

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or. Probes that have a high fluorescence quantum yield when bound to a particular target but are otherwise effectively nonfluorescent yield extremely low reagent background signals.

The ultrasensitive SYBR. (hbk.), (hbk.), (e-book), X (e-book) Contents Basic principles of fluorescence and energy transfer / Larry E. Morrison. Nucleic acid-based fluorescent probes and their analytical fluorescent derivative of the target [ ], or the fluorescent aptamer may serve as a reporter sensor for detection ofAuthor: Bernard Juskowiak.

Highly fluorescent nucleotide base analogs provide sensitive probes for studying the structure, dynamics and interactions of nucleic acids. Fluorescent Nucleotide Base Analogs as Probes of Nucleic Acid Structure, Cited by: Fluorescent labeling is the process of covalently attaching a fluorophore to another molecule, such as a protein or nucleic acid.

This is generally accomplished by using a reactive derivative of the fluorophore. The use of fluorescent nucleic acid base analogues is becoming increasingly important in the fields of biology, biochemistry and biophysical chemistry as well as in the field of DNA Cited by: The DNA base substitute approach by the (S)amino-1,2-propanediol linker allows placing two fluorophores in a precise way inside a given DNA framework.

The double helical architecture around Cited by:. Fluorescence resonance energy transfer (FRET) strategy has been widely applied in designing ratiometric probes for bioimaging applications. Unfortunately, for FRET systems, sufficiently large .Nucleic acid detection methods include SYBR green, fluorescence resonance energy transfer (FRET) system and hydrolysis (TaqMan) probes.

Other amplification methods described in the chapter are Cited by: 1.Fluorescence to Study Nucleic Acids fluorescence resonance energy transfer can detect nucleic acid hybridization in solution.

modified fluorescent-labeled oligonucleotide probes in the.